See main article: 2010 ECM Prague Open.
The women's singles of the 2010 ECM Prague Open tournament was played on clay in Prague, Czech Republic.
Sybille Bammer was the defending champion, but chose not to participate.
Ágnes Szávay defeated Barbora Záhlavová-Strýcová in the final (6–2, 1–6, 6–2).