The 2010 Due Ponti Cup was a professional tennis tournament played on outdoor red clay courts. It was part of the 2010 ATP Challenger Tour. It took place in Rome, Italy between 31 May and 6 June 2010.
| Nationality | Player | Ranking* | Seeding | |
|---|---|---|---|---|
 Brazil | Ricardo Mello | 90 | 1 | |
 Italy | Paolo Lorenzi | 95 | 2 | |
| Italy | Filippo Volandri | 118 | 3 | |
| Brazil | João Souza | 125 | 4 | |
| Italy | Simone Bolelli | 126 | 5 | |
 Argentina | Máximo González | 148 | 6 | |
 Romania | Victor Crivoi | 158 | 7 | |
| Romania | Adrian Ungur | 166 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry into the singles main draw as an alternate:
The following players received entry from the qualifying draw:
The following player received the lucky loser spot:
See main article: 2010 Due Ponti Cup – Singles. Filippo Volandri def. 
Reda El Amrani, 6–3, 6–2
See main article: 2010 Due Ponti Cup – Doubles. 
Santiago González / 
Travis Rettenmaier def. 
Sadik Kadir / 
Purav Raja, 6–2, 6–4
