See main article: 2010 Copa Sudamericana.
The preliminary stages of the 2010 Copa Sudamericana de Clubes are:
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----Colo-Colo 3–3 Universitario on points. Universitario advanced on away goals.
----River Plate 3–3 Guaraní on points. Guaraní advanced on away goals.
----Barcelona advanced on points 6–0.
----Atlético Huila advanced on points 4–1.
----Oriente Petrolero advanced on points 4–1.
----Defensor Sporting advanced on points 4–1.
----Universidad San Martín 3–3 Deportivo Quito on points. Universidad San Martín advanced on away goals.
----Santa Fe 3–3 Deportivo Lara on points. Santa Fe advanced on goal difference.
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----San José advanced on points 4-1.
----Independiente advanced on points 4–1.
----Peñarol advanced on points 6–0.
----Palmeiras 3–3 Vitória on points. Palmeiras advanced on goal difference.
----Santa Fe advanced on points 4–1.
----Avaí 3–3 Santos on points. Avaí advanced on goal difference.
----Deportes Tolima 3–3 Oriente Petrolero on points. Deportes Tolima advanced on goal difference.
----Unión San Felipe 3–3 Guaraní on points. Unión San Felipe advanced 8–7 on penalties.
----Banfield advanced on points 4–1.
----Emelec 3–3 Universidad San Martín on points. Emelec advanced on goal difference.
----Atlético Mineiro advanced on points 4–1.
----Universitario advanced on points 4–1.
----Goiás advanced on points 4–1.
Note: This is the largest margin of victory in the history of the Copa Sudamericana.----Sport Huancayo 3–3 Defensor Sporting on points. Defensor Sporting advanced on goal difference.
----Newell's Old Boys advanced on points 4–1.