The 2010 Copa Petrobras Santiago was a professional tennis tournament played on hard courts. It was the fourth edition of the tournament which was part of the 2010 ATP Challenger Tour. It took place in Santiago, Chile between 18 and 24 October 2010.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Italy | Fabio Fognini | 63 | 1 | |
 Spain | Pere Riba | 80 | 2 | |
| Spain | Rubén Ramírez Hidalgo | 87 | 3 | |
 Brazil | João Souza | 105 | 4 | |
| Spain | Albert Ramos Viñolas | 121 | 5 | |
| Rui Machado | 122 | 6 | ||
 Kazakhstan | Yuri Schukin | 123 | 7 | |
| Nicolás Massú | 128 | 8 |
The following players received wildcards into the singles main draw:
The following players received a special entrant into the singles main draw:
The following players received an alternate into the singles main draw:
The following players received entry from the qualifying draw:
Juan Pablo Brzezicki
Axel Michon Maxime Teixeira
Goran TošićSee main article: 2010 Copa Petrobras Santiago – Singles. Fabio Fognini def. Paul Capdeville, 6–2, 7–6(2)
See main article: 2010 Copa Petrobras Santiago – Doubles. Daniel Muñoz de la Nava / Rubén Ramírez Hidalgo def. 
Nikola Ćirić / Goran Tošić, 6–4, 6–2