The 2010 Copa Petrobras São Paulo was a professional tennis tournament played on outdoor red clay courts. It was the seventh edition of the tournament which is part of the 2010 ATP Challenger Tour. It took place in São Paulo, Brazil between 25 and 31 October 2010.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Brazil | Thomaz Bellucci | 27 | 1 | |
 Spain | Rubén Ramírez Hidalgo | 86 | 2 | |
| Rui Machado | 106 | 3 | ||
| Brazil | João Souza | 107 | 4 | |
| Spain | Albert Ramos-Viñolas | 123 | 5 | |
| Nicolás Massú | 125 | 6 | ||
 Kazakhstan | Yuri Schukin | 127 | 7 | |
| Spain | Daniel Muñoz de la Nava | 134 | 8 |
The following players received wildcards into the singles main draw:
Thomaz Bellucci Tiago Fernandes
Christian Lindell Augusto MeirellesThe following players received a special entry into the singles main draw:
The following players received entry from the qualifying draw:
Thomas Cazes-Carrère Nicolas Devilder Tiago Lopes Maxime TeixeiraThe following player received entry as a Lucky loser into the singles main draw:
Eládio Ribeiro NetoSee main article: 2010 Copa Petrobras São Paulo – Singles. Marcos Daniel def. Thomaz Bellucci, 6–1, 3–6, 6–3
See main article: 2010 Copa Petrobras São Paulo – Doubles. Franco Ferreiro / André Sá def. Rui Machado / Daniel Muñoz de la Nava, 3–6, 7–6(2), [10–8]