| Date: | August 30 – September 5 |
| Edition: | 5th |
| Location: | Como, Italy |
| Champs: | Robin Haase |
| Champd: | Frank Moser /  David Škoch |
The 2010 Città di Como Challenger was a professional tennis tournament played on outdoor clay courts. It was the fifth edition of the tournament which is part of the 2010 ATP Challenger Tour. It took place in Como, Italy between August 30 and September 5, 2010.
| Nationality | Player | Ranking* | Seeding | |
|---|---|---|---|---|
| Robin Haase | 97 | 1 | ||
 Italy | Filippo Volandri | 101 | 2 | |
| Italy | Paolo Lorenzi | 104 | 3 | |
| Julian Reister | 106 | 4 | ||
 Belgium | Steve Darcis | 110 | 5 | |
 Spain | Albert Ramos-Viñolas | 119 | 6 | |
 Romania | Adrian Ungur | 122 | 7 | |
| Rui Machado | 126 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry as an alternate into the singles main draw:
The following players received entry as a Special Exempt into the singles main draw:
The following players received entry from the qualifying draw:
See main article: 2010 Città di Como Challenger – Singles. Robin Haase def. Ivo Minář, 6–4, 6–3
See main article: 2010 Città di Como Challenger – Doubles. Frank Moser / David Škoch def. Martin Emmrich / 
Mateusz Kowalczyk, 5–7, 7–6(2), [10–5]
