The 2010 Città di Caltanissetta was a professional tennis tournament played on outdoor red clay courts. It was part of the 2010 ATP Challenger Tour. It took place in Caltanissetta, Italy between 15 and 21 March 2010.
| Nationality | Player | Ranking* | Seeding | |
|---|---|---|---|---|
 Spain | Santiago Ventura | 91 | 1 | |
 Italy | Paolo Lorenzi | 92 | 2 | |
| Spain | Pere Riba | 117 | 3 | |
| Spain | Iván Navarro | 123 | 4 | |
| Spain | Rubén Ramírez Hidalgo | 148 | 5 | |
| Grega Žemlja | 149 | 6 | ||
| Spain | David Marrero | 150 | 7 | |
 Czech Republic | Ivo Minář | 151 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
The following player received special exempt into the main draw:
See main article: 2010 Città di Caltanissetta – Singles. Robin Haase def. Matteo Trevisan, 7–5, 6–3
See main article: 2010 Città di Caltanissetta – Doubles. David Marrero / Santiago Ventura def. 
Uladzimir Ignatik / 
Martin Kližan, 7–6(3), 6–4
