| Date: | March 22 – March 28 |
| Edition: | 4th |
| Category: | ATP Challenger Tour |
| Surface: | Hard (indoor) |
| Location: | Rimouski, Canada |
| Venue: | Tennis de Rimouski |
| Champs: | Rik de Voest |
| Champd: |  Kaden Hensel / Adam Hubble |
| Before Year: | 2008 |
| Main Name: | Challenger de Rimouski |
The 2010 Challenger Banque Nationale de Rimouski was a professional tennis tournament played on indoor hard courts. It was the 4th edition of the tournament and part of the 2010 ATP Challenger Tour, offering a total of $35,000 in prize money. It took place in Rimouski, Canada between March 22 and March 28, 2010.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Poland | Michał Przysiężny | 137 | 1 | |
| Dieter Kindlmann | 174 | 2 | ||
| Alex Kuznetsov | 184 | 3 | ||
| Michael Lammer | 191 | 4 | ||
| Brendan Evans | 198 | 5 | ||
 Korea, Republic of | Im Kyu-tae | 202 | 6 | |
 France | Vincent Millot | 208 | 7 | |
| Lester Cook | 215 | 8 |
The following players received wildcards into the singles main draw:
Philip Bester Érik Chvojka Milos Raonic Zachary WhiteThe following players received entry from the qualifying draw:
See main article: 2010 Challenger Banque Nationale de Rimouski – Singles. Rik de Voest def. 
Tim Smyczek, 6–0, 7–5
See main article: 2010 Challenger Banque Nationale de Rimouski – Doubles. Kaden Hensel / Adam Hubble def. Scott Lipsky / David Martin, 7–6(7–5), 3–6, [11–9]
