| Challenge Trophy | |
| Year: | 2010 |
| Other Titles: | Canadian National Challenge Cup |
| Country: | Canada |
| Num Teams: | 10 |
| Prevseason: | 2009 |
| Nextseason: | 2011 |
The 2010 Challenge Trophy was hosted in Charlottetown, Prince Edward Island on 6 to 10 October 2010. Defending champion Hellas SC of Manitoba failed to qualify as they lost to Lucania SC in the semi-final round in the Manitoba play down.
The seeding is based on the results from the previous year. New Brunswick did not send a team this year so the host gained a second entry.
Group 1
1. Winnipeg Lucania FC
4. Calgary Dinosaurs
5. Gorge FC
8. Huskie Alumni
9. AEK London FC
Group 2
2. Royal Select de Beauport
3. 1 Churchill Arms
6. Holy Cross Kirby
7. Halifax Dunbrack
10. 2 PEI Selects
See main article: Gorge FC. In the provincial final, Karpati's squad
See main article: Churchill Arms FC.
The following are details of each of the provincial finals that will determine each representative. New Brunswick is serving a suspension, so PEI as host will get 2 teams. List of Challenge Trophy 2010 teams