| Sport: | football |
| Year: | 2010 |
| Team: | CSU Pueblo ThunderWolves |
| Conference: | Rocky Mountain Athletic Conference |
| Short Conf: | RMAC |
| Record: | 9–2 |
| Conf Record: | 7–2 |
| Head Coach: | John Wristen |
| Hc Year: | 3rd |
| Def Coach: | Hunter Hughes |
| Dc Year: | 3rd |
The 2010 CSU Pueblo ThunderWolves football team represented Colorado State University–Pueblo as a member of the Rocky Mountain Athletic Conference (RMAC) during 2010 NCAA Division II football season. Led by third-year head coach John Wristen, the ThunderWolves compiled an overall record of 9–2 with a mark of 7–2 in conference play, tying for third place in the RMAC. CSU Pueblo played their home games at Neta and Eddie DeRose ThunderBowl in Pueblo, Colorado.