See main article: 2010 CAF Champions League. This page provides the summaries of the matches of the qualifying rounds for the group stage of the 2010 CAF Champions League.
This is a knockout stage of the 52 teams that did not receive byes to the first round.
First legs: 12–14 February 2010; Second legs: 26–28 February 2010.
----
APR won 3 - 2 on aggregate and advanced to the first round.
----
Djoliba won 1 - 0 on aggregate and advanced to the first round.
----
Aggregate 2 - 2. ASC Linguère won the penalty shootout and advanced to the first round.
----
Ismaily won 2 - 0 on aggregate and advanced to the first round.
----
US Stade Tamponnaise won 5 - 2 on aggregate and advanced to the first round.
----
Africa Sports advanced to the first round after the Benin representative was withdrawn.
----
Raja Casablanca won 4 - 2 on aggregate and advanced to the first round.
----
Petro de Luanda won 9 - 3 on aggregate and advanced to the first round.
----
Aggregate 2 - 2. Club Africain advanced on the away goals rule to the first round.
----
JS Kabylie won 5 – 1 on aggregate and advanced to the first round.
----
Gazelle FC won 3 - 2 on aggregate and advanced to the first round.
----
Al-Merreikh won 4 - 2 on aggregate and advanced to the first round.
----
Espérance ST won 5 - 4 on aggregate and advanced to the first round.
----
ASFA Yennega won 6 - 1 on aggregate and advanced to the first round.
----
Curepipe Starlight won 3 - 2 on aggregate and advanced to the first round.
----
Aggregate 2 - 2. Gaborone United advanced on the away goals rule to the first round.
----
Saint Eloi Lupopo won 4 - 2 on aggregate and advanced to the first round.
----
Gunners won 6 - 1 on aggregate and advanced to the first round.
----
Ittihad won 8 - 1 on aggregate and advanced to the first round.
----
Difaa El Jadida won 3 - 0 on aggregate and advanced to the first round.
----
Zanaco won 4 - 1 on aggregate and advanced to the first round.
----
Union Douala advanced to the first round after the São Tomé and Príncipe representative was withdrawn.
----
ES Sétif won 4 - 3 on aggregate and advanced to the first round.
----
Aggregate 4 - 4. Tiko United won the penalty shootout and advanced to the first round.
----
Supersport United won 5 - 3 and advanced to the first round.
----
Ferroviário Maputo won 9 - 4 on aggregate and advanced to the first round.
----
This is a knock-out stage of 32 teams; the 26 teams advancing from the preliminary round, and 6 teams that received byes to this round.
First legs: 19–21 March 2010; Second legs: 2–5 April 2010.
----
TP Mazembe won 2 - 1 on aggregate and advanced to the second round.
----
Aggregate 1 - 1. Djoliba won the penalty shootout and advanced to the second round.
----
Ismaily won 3 - 2 on aggregate and advanced to the second round.
----
Al-Hilal Omdurman won 4 - 1 on aggregate and advanced to the second round.
----
Petro de Luanda won 2 - 1 on aggregate and advanced to the second round.
---- JS Kabylie won 2 - 1 on aggregate and advanced to the second round.
---- Al-Merreikh won 3 - 1 on aggregate and advanced to the second round.
---- Espérance ST won 7 - 2 on aggregate and advanced to the second round.
---- Gaborone United won 6 - 0 on aggregate and advanced to the second round.
---- Dynamos won 2 - 0 on aggregate and advanced to the second round.
---- Al-Ahly won 2 - 1 on aggregate and advanced to the second round.
----
Aggregate 2 - 2. Ittihad won the penalty shootout and advanced to the second round.
---- Zanaco won 2 - 1 on aggregate and advanced to the second round.
---- ES Sétif won 7 - 0 on aggregate and advanced to the second round.
---- Aggregate 3 - 3. Heartland advanced on the away goals rule to the second round.
---- Supersport United won 3 - 2 on aggregate and advanced to the second round.----
This is a knock-out stage of the 16 teams that advanced from the first round; winners will advance to the group stage, with the losers advancing to the CAF Confederation Cup.
First legs: 23–25 April 2010; Second legs 7–9 May 2010.
---- TP Mazembe won 4 – 0 on aggregate and advanced to the group stage.
---- Ismaily won 4 – 1 on aggregate and advanced to the group stage.
---- JS Kabylie won 3 – 2 on aggregate and advanced to the group stage.
---- Espérance ST won 4 - 1 on aggregate and advanced to the group stage.
---- Dynamos won 4 - 2 on aggregate and advanced to the group stage.
---- Al-Ahly won 3 – 2 on aggregate and advanced to the group stage.
---- ES Sétif won 3 - 2 on aggregate and advanced to the group stage.
---- Heartland won 4 – 2 on aggregate and advanced to the group stage.