See main article: 2010 Brisbane International.
Victoria Azarenka was the defending champion, but chose not to participate this year.
Kim Clijsters won in the final 6–3, 4–6, 7–6(8–6), against her compatriot Justine Henin, saving two match points in the third set. Henin marked her return from the tournament after her first retirement.
Kim Clijsters (champion)
Nadia Petrova (first round)
Ana Ivanovic (semifinals)
Daniela Hantuchová (quarterfinals) Alisa Kleybanova (first round)
Aleksandra Wozniak (second round)
Melinda Czink (quarterfinals)
Iveta Benešová (first round)