The 2010 Barcelona Ladies Open was a tennis tournament played on outdoor clay courts. It was the 4th edition of the Barcelona Ladies Open, and an International-level tournament on the 2010 WTA Tour. It took place at the David Lloyd Club Turó in Barcelona, Catalonia, Spain, from April 10 through April 17, 2010.
| Athlete | Nationality | Ranking* | Seeding | |
|---|---|---|---|---|
| Francesca Schiavone |  Italy | 17 | 1 | |
| Aravane Rezaï |  France | 20 | 2 | |
| María José Martínez Sánchez |  Spain | 28 | 3 | |
| Maria Kirilenko |  Russia | 31 | 4 | |
| Sorana Cîrstea |  Romania | 36 | 5 | |
| Gisela Dulko |  Argentina | 37 | 6 | |
| Carla Suárez Navarro | Spain | 38 | 7 | |
| Lucie Šafářová |  Czech Republic | 40 | 8 |
The following players received wildcards into the main draw:
The following players received entry from the qualifying draw:
See main article: 2010 Barcelona Ladies Open – Singles. Francesca Schiavone def. Roberta Vinci, 6–1, 6-1
See main article: 2010 Barcelona Ladies Open – Doubles. Sara Errani / Roberta Vinci def. Timea Bacsinszky / Tathiana Garbin, 6–1, 3–6, [10–2]