The 2010 Bancolombia Open is a professional tennis tournament played on indoor red clay courts. It is part of the 2010 ATP Challenger Tour. It took place in Bogotá, Colombia between 5 and 12 April 2010.
| Nationality | Player | Ranking* | Seeding | |
|---|---|---|---|---|
 Colombia | Alejandro Falla | 70 | 1 | |
| Colombia | Santiago Giraldo | 88 | 2 | |
 Italy | Paolo Lorenzi | 90 | 3 | |
 Ecuador | Nicolás Lapentti | 102 | 4 | |
 Brazil | Ricardo Mello | 106 | 5 | |
| Brazil | Thiago Alves | 123 | 6 | |
| Kevin Kim | 128 | 7 | ||
| Brazil | João Souza | 156 | 8 |
The following players received wildcards into the singles main draw:
Juan Sebastián Cabal
Carlton Fiorentino Juan Sebastián Gómez Eduardo StruvayThe following players received entry from the qualifying draw:
Martín Alund
Attila Balázs
Arnau Brugués-Davi Guido PellaSee main article: 2010 Bancolombia Open – Singles. João Souza def. Alejandro Falla, 4 - 6, 6 - 4, 6 - 1
See main article: 2010 Bancolombia Open – Doubles. Franco Ferreiro / 
Santiago González def. Dominik Meffert / 
Philipp Oswald, 6–3, 5–7, [10–7]