| Champ: | Korie Homan |
| Runner: |  Florence Gravellier |
| Score: | 6 - 2, 6 - 2 |
See main article: 2010 Australian Open.
Korie Homan defeated Florence Gravellier in the final, 6 - 2, 6 - 2 to win the women's singles wheelchair tennis title at the 2010 Australian Open.
Esther Vergeer was the four-time reigning champion, but did not participate this year.
Florence Gravellier (final)