The 2010 Alessandria Challenger (known as 2010 Trofeo Cassa di Risparmio Alessandria due to sponsorship) was a professional tennis tournament played on outdoor red clay courts. It was part of the 2010 ATP Challenger Tour. It took place in Alessandria, Italy between May 24–30, 2010.
| Nationality | Player | Ranking* | Seeding | |
|---|---|---|---|---|
| Frederico Gil | 100 | 1 | ||
| Rui Machado | 106 | 2 | ||
| Björn Phau | 115 | 3 | ||
 Austria | Stefan Koubek | 118 | 4 | |
 Spain | Rubén Ramírez Hidalgo | 119 | 5 | |
 Brazil | João Souza | 128 | 6 | |
 Kazakhstan | Mikhail Kukushkin | 137 | 7 | |
 Romania | Victor Crivoi | 159 | 8 |
The following players received wildcards into the singles main draw:
Laurynas Grigelis
Jacopo Marchegiani Marco Crugnola Matteo TrevisanThe following players received special exempt into the singles main draw:
The following players received entry into the singles main draw with a protected ranking:
The following players received entry from the qualifying draw:
See main article: 2010 Alessandria Challenger – Singles. Björn Phau def. 
Carlos Berlocq, 7–6(6), 2–6, 6–2
See main article: 2010 Alessandria Challenger – Doubles. Ivan Dodig / Lovro Zovko def. Marco Crugnola / Daniel Muñoz de la Nava, 6–4, 6–4
