| Score: | 7–5, 6–3 |
| Draw: | 48 (5) |
| Seeds: | 16 |
See main article: 2009 Wimbledon Championships. Bob Bryan and Samantha Stosur were the defending champions but lost in the quarterfinals to Mark Knowles and Anna-Lena Grönefeld.
Knowles and Grönefeld defeated Leander Paes and Cara Black in the final, 7–5, 6–3 to win the mixed doubles tennis title at the 2009 Wimbledon Championships.[1]
All seeds received a bye into the second round.
See also: 1 and 1. 
Leander Paes / Cara Black (final)
See also: 2 and 4. 
Bob Bryan / 
Samantha Stosur (quarterfinals)
See also: 3 and 3. 
Marcin Matkowski / Lisa Raymond (third round)
See also: 4 and 2. Kevin Ullyett / Hsieh Su-wei (quarterfinals)
See also: 5 and 4. 
Daniel Nestor / 
Elena Vesnina (third round)
See also: 6 and 3. Mike Bryan / Bethanie Mattek-Sands (third round)
See also: 7 and 1. 
Robert Lindstedt / Rennae Stubbs (third round)
See also: 8 and 2. 
Max Mirnyi / Nadia Petrova (third round)
See also: 9 and 4. Mark Knowles / Anna-Lena Grönefeld (champions)
See also: 10 and 3. 
Nenad Zimonjić / 
Yan Zi (second round)
See also: 11 and 1. 
André Sá / 
Ai Sugiyama (quarterfinals)
See also: 12 and 2. Stephen Huss / 
Virginia Ruano Pascual (semifinals)
See also: 13 and 1. Mahesh Bhupathi / Sania Mirza (third round)
See also: 14 and 4. Marcelo Melo / Peng Shuai (second round)
See also: 15 and 3. 
Lukáš Dlouhý / Iveta Benešová (quarterfinals)
See also: 16 and 2. Christopher Kas / Chuang Chia-jung (third round)