The 2009 Tunis Open was a professional tennis tournament played on outdoor red clay courts. It was part of the Tretorn SERIE+ of the 2009 ATP Challenger Tour. It took place in Tunis, Tunisia between April 27 and March 3, 2009.
| Nationality | Player | Ranking* | Seeding | |
|---|---|---|---|---|
 Finland | Jarkko Nieminen | 50 | 1 | |
 France | Nicolas Devilder | 64 | 2 | |
| Björn Phau | 65 | 3 | ||
| Frederico Gil | 75 | 4 | ||
 Argentina | Diego Junqueira | 77 | 5 | |
 Spain | Pablo Andújar | 91 | 6 | |
| Wayne Odesnik | 98 | 7 | ||
| Argentina | Leonardo Mayer | 105 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
See main article: 2009 Tunis Open – Singles. Gastón Gaudio def. Frederico Gil, 6–2, 1–6, 6–3
See main article: 2009 Tunis Open – Doubles. Brian Dabul / Leonardo Mayer def. 
Johan Brunström / Jean-Julien Rojer, 6–4, 7–6(6)
