The 2009 Tennislife Cup was a professional tennis tournament played on outdoor red clay courts. It was the third edition of the tournament which was part of the 2009 ATP Challenger Tour. It took place in Naples, Italy between 28 September and 4 October 2009.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Australia | Peter Luczak | 70 | 1 | |
 Spain | Óscar Hernández | 76 | 2 | |
| Spain | Marcel Granollers | 79 | 3 | |
 Russian Federation | Teymuraz Gabashvili | 80 | 4 | |
 Italy | Potito Starace | 91 | 5 | |
| Frederico Gil | 102 | 6 | ||
| Lamine Ouahab | 114 | 7 | ||
 Brazil | Thiago Alves | 116 | 8 |
The following players received wildcards into the singles main draw:
Enrico Fioravante Giancarlo Petrazzuolo
Adrian Ungur Filippo VolandriThe following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
Alberto Brizzi
Reda El Amrani Sergio Gutiérrez-FerrolThe following players received a lucky loser entry:
See main article: 2009 Tennislife Cup – Singles. Frederico Gil def. Potito Starace, 2–6, 6–1, 6–4
See main article: 2009 Tennislife Cup – Doubles. Frederico Gil / Ivan Dodig def. Thiago Alves / 
Lukáš Rosol, 6–1, 6–3