The 2009 Tashkent Challenger was a professional tennis tournament played on indoor hard courts. It was the second edition of the tournament which was part of the 2009 ATP Challenger Tour. It took place in Tashkent, Uzbekistan between 12 and 18 October 2009.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Lu Yen-hsun | 76 | 1 | ||
 Uzbekistan | Denis Istomin | 89 | 2 | |
 Cyprus | Marcos Baghdatis | 90 | 3 | |
 Belgium | Christophe Rochus | 93 | 4 | |
 Russian Federation | Teymuraz Gabashvili | 102 | 5 | |
| Rajeev Ram | 103 | 6 | ||
 India | Somdev Devvarman | 129 | 7 | |
 Ukraine | Sergiy Stakhovsky | 133 | 8 |
The following players received wildcards into the singles main draw:
Rifat Biktyakov Rohan Bopanna Murad Inoyatov Vaja UzakovThe following players received entry from the qualifying draw:
Arsen Asanov (as a Lucky loser)
Andrei Gorban Sarvar Ikramov Konstantin Kravchuk
Jiří KrkoškaSee main article: 2009 Tashkent Challenger – Singles. Marcos Baghdatis def. Denis Istomin, 6–3, 1–6, 6–3
See main article: 2009 Tashkent Challenger – Doubles. Murad Inoyatov / Denis Istomin def. Jiří Krkoška / 
Lukáš Lacko, 7–6(4), 6–4