The 2009 Tallahassee Tennis Challenger was a professional tennis tournament played on outdoor hard courts. It was part of the 2009 ATP Challenger Tour. It took place in Tallahassee, Florida, United States between April 20 and April 26, 2009.
| Nationality | Player | Ranking* | Seeding | |
|---|---|---|---|---|
| Bobby Reynolds | 67 | 1 | ||
| Robert Kendrick | 82 | 2 | ||
| Kevin Kim | 104 | 3 | ||
| Vince Spadea | 109 | 4 | ||
 Canada | Frank Dancevic | 112 | 5 | |
 Japan | Go Soeda | 117 | 6 | |
| John Isner | 126 | 7 | ||
| Jesse Levine | 132 | 8 |
The following players received wildcards into the singles main draw:
Jean-Yves Aubone Taylor Dent Michael McClune Tim SmyczekThe following players received entry from the qualifying draw:
The following players received special exempt into the singles main draw:
See main article: article and 2009 Tallahassee Tennis Challenger – Singles. John Isner def. Donald Young, 7–5, 6–4
See main article: article and 2009 Tallahassee Tennis Challenger – Doubles. Eric Butorac / Scott Lipsky def. 
Colin Fleming / Ken Skupski, 6–1, 6–4
