The 2009 Royal Bank of Scotland Challenger was a professional tennis tournament played on outdoor hard courts. It was the third edition of the tournament which was part of the 2009 ATP Challenger Tour. It took place in Tiburon, California, United States between October 12 and October 18, 2009. It was part of the Tiburon Challenger series.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Michael Russell | 91 | 1 | ||
| Kevin Kim | 101 | 2 | ||
| Jesse Levine | 113 | 3 | ||
 Colombia | Santiago Giraldo | 114 | 4 | |
| Roko Karanušić | 132 | 5 | ||
| Donald Young | 155 | 6 | ||
| Grega Žemlja | 156 | 7 | ||
| Colombia | Carlos Salamanca | 158 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
Nick Lindahl
Louk Sorensen
Artem Sitak (as a Lucky loser)See main article: 2009 Royal Bank of Scotland Challenger – Singles. 
Go Soeda def. 
Ilija Bozoljac, 3–6, 6–3, 6–2
See main article: 2009 Royal Bank of Scotland Challenger – Doubles. Treat Conrad Huey / 
Harsh Mankad def. Ilija Bozoljac / Dušan Vemić, 6–4, 6–4
