The 2009 Roma Open was a professional tennis tournament played on outdoor red clay courts. It was part of the 2009 ATP Challenger Tour. It took place in Rome, Italy between 20 and 26 April 2009.
| Nationality | Player | Ranking* | Seeding | |
|---|---|---|---|---|
 Argentina | ||||
 Uzbekistan | Denis Istomin | 100 | 2 | |
 Austria | Daniel Köllerer | 103 | 3 | |
| Argentina | Brian Dabul | 106 | 4 | |
| Simon Greul | 113 | 5 | ||
 Romania | Victor Crivoi | 116 | 6 | |
 Czech Republic | Jiří Vaněk | 122 | 7 | |
 France | Adrian Mannarino | 127 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
Francesco Aldi
Javier Genaro-Martínez
Tim GoranssonThe following players received lucky loser into the singles main draw:
Attila Balázs
Robert Smeets Federico TorresiSee main article: 2009 Roma Open – Singles. Daniel Köllerer def. Andreas Vinciguerra, 6–3, 6–3.
See main article: 2009 Roma Open – Doubles. Simon Greul / Christopher Kas def. Johan Brunström / Jean-Julien Rojer, 4–6, 7–6(2), [10–2].
