The 2009 Rai Open was a professional tennis tournament played on outdoor red clay courts. It was part of the 2009 ATP Challenger Tour. It took place in Rome, Italy between 13 and 18 April 2009.
| Nationality | Player | Ranking* | Seeding | |
|---|---|---|---|---|
 Argentina | Sergio Roitman | 98 | 1 | |
| Simon Greul | 112 | 2 | ||
| Roko Karanušić | 122 | 3 | ||
 Czech Republic | Jiří Vaněk | 123 | 4 | |
 Serbia | Ilija Bozoljac | 134 | 5 | |
| Czech Republic | Lukáš Rosol | 137 | 6 | |
 Italy | Filippo Volandri | 140 | 7 | |
 France | Alexandre Sidorenko | 155 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
Andrea Arnaboldi Thierry Ascione
Eric Gomes Giancarlo Petrazzuolo Stefano Galvani (as a Lucky loser)The following player received special exempt into the main draw:
See main article: 2009 Rai Open – Singles. Sebastián Decoud def. Simon Greul, 7–6(2), 6–1
See main article: 2009 Rai Open – Doubles. Simon Greul / Alessandro Motti def. Daniele Bracciali / Filippo Volandri, 6–4, 7–5