See main article: 2009 Mutua Madrileña Madrid Open.
Dinara Safina defeated Caroline Wozniacki in the final, 6–2, 6–4 to win the inaugural women's singles tennis title at the 2009 Madrid Open.
The four Rome semifinalists received a bye into the second round. They were as follows:
Dinara Safina (champion)
Venus Williams (second round) Svetlana Kuznetsova (second round)
Victoria Azarenka (third round)