The 2009 Mamaia Challenger was a professional tennis tournament played on outdoor red clay courts. It was part of the 2009 ATP Challenger Tour. It took place in Constanța, Romania between 22 and 28 June 2009.
| Nationality | Player | Ranking* | Seeding | |
|---|---|---|---|---|
 Italy | Marco Crugnola | 176 | 1 | |
| Italy | Alessio di Mauro | 181 | 2 | |
 Spain | Pablo Santos | 185 | 3 | |
| Blaž Kavčič | 186 | 4 | ||
 Serbia | Boris Pašanski | 196 | 5 | |
| Thiemo de Bakker | 198 | 6 | ||
| Dieter Kindlmann | 204 | 7 | ||
| Julian Reister | 223 | 8 |
The following players received wildcards into the singles main draw:
Alin-Mihai Constantin Adrian-Marin Dăncescu Andrei Mlendea Andrei SăvulescuThe following players received entry from the qualifying draw:
See main article: 2009 Mamaia Challenger – Singles. Blaž Kavčič def. Julian Reister, 3–6, 6–3, 6–4
See main article: 2009 Mamaia Challenger – Doubles. Adrián García / David Marrero def. Adrian Cruciat / Florin Mergea, 7–6(5), 6–2
