The 2009 Lima Challenger was a professional tennis tournament played on outdoor red clay courts. It was the sixth edition of the tournament which was part of the 2009 ATP Challenger Tour. It took place in Lima, Peru between 16 and 22 November 2009.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Argentina | Eduardo Schwank | 146 | 1 | |
 Brazil | Júlio Silva | 147 | 2 | |
| Brazil | João Souza | 165 | 3 | |
| Argentina | Gastón Gaudio | 171 | 4 | |
| Argentina | Brian Dabul | 183 | 5 | |
| Argentina | Diego Junqueira | 192 | 6 | |
| Argentina | Juan-Martín Aranguren | 195 | 7 | |
| Jorge Aguilar | 244 | 8 |
The following players received wildcards into the singles main draw:
Duilio Beretta Francisco Carbajal Sergio Galdós Iván MirandaThe following players received entry from the qualifying draw:
See main article: 2009 Lima Challenger – Singles. Eduardo Schwank def. Jorge Aguilar, 7–5, 6–4
See main article: 2009 Lima Challenger – Doubles. Martín Alund / Juan-Martín Aranguren def. Cristóbal Saavedra-Corvalán / Guillermo Rivera Aránguiz, 6–4, 6–4