The 2009 Košice Open was a professional tennis tournament played on outdoor red clay courts. It was part of the 2009 ATP Challenger Tour. It took place in Košice, Slovakia between 8 and 14 June 2009.
| Nationality | Player | Ranking* | Seeding | |
|---|---|---|---|---|
 Spain | Santiago Ventura | 122 | 1 | |
| Spain | Rubén Ramírez Hidalgo | 126 | 2 | |
 Czech Republic | Jiří Vaněk | 155 | 3 | |
| Spain | David Marrero | 164 | 4 | |
 Slovakia | Dominik Hrbatý | 165 | 5 | |
 Kazakhstan | Yuri Schukin | 176 | 6 | |
 Argentina | Juan Pablo Brzezicki | 177 | 7 | |
| Spain | Miguel Ángel López Jaén | 178 | 8 |
The following players received wildcards into the singles main draw:
Jerzy Janowicz Martin Kližan Miloslav Mečíř Jr.
Nicolas ReissigThe following players received entry a special Exempt into the singles main draw:
The following players received entry from the qualifying draw:
Diego Álvarez Kamil Čapkovič
Ádám Kellner Marek Semjan (as a Lucky loser)
Ivan SergeyevSee main article: 2009 Košice Open – Singles. 
Stéphane Robert def. Jiří Vaněk, 7–6(5), 7–6(5)
See main article: 2009 Košice Open – Doubles. Rubén Ramírez Hidalgo / Santiago Ventura def. Dominik Hrbatý / Martin Kližan, 6–2, 7–6(5)