| Date: | August 17 – August 23 |
| Edition: | 3rd |
| Location: | Qarshi, Uzbekistan |
| Champs: |  Rainer Eitzinger |
| Champd: |  Sadik Kadir /  Purav Raja |
The 2009 Karshi Challenger was a professional tennis tournament played on outdoor hard courts. It was the third edition of the Karshi Challenger tournament which was part of the 2009 ATP Challenger Tour. It took place in Qarshi, Uzbekistan between 17 and 23 August 2009.
| Nationality | Player | Ranking* | Seeding | |
|---|---|---|---|---|
 Russian Federation | Michail Elgin | 193 | 1 | |
 Ukraine | Illya Marchenko | 202 | 2 | |
 Slovakia | Kamil Čapkovič | 221 | 3 | |
 Spain | Carles Poch-Gradin | 258 | 4 | |
| Andis Juška | 270 | 5 | ||
| Ukraine | Ivan Sergeyev | 274 | 6 | |
| Deniss Pavlovs | 297 | 7 | ||
| Russian Federation | Valery Rudnev | 300 | 8 |
The following players received wildcards into the singles main draw:
Rifat Biktyakov Jakhongir Jalalov Sergey Shipilov Vaja UzakovThe following players received entry from the qualifying draw:
Ričardas Berankis Murad Inoyatov Sadik Kadir Mikhail LedovskikhSee main article: 2009 Karshi Challenger – Singles. Rainer Eitzinger def. Ivan Sergeyev, 6–3, 1–6, 7–6(3)
See main article: 2009 Karshi Challenger – Doubles. Sadik Kadir / Purav Raja def. Andis Juška / Deniss Pavlovs, 6–3, 7–6(4)