See main article: 2009 Italian Open (tennis).
Dinara Safina defeated Svetlana Kuznetsova in the final, 6–3, 6–2 to win the women's singles tennis title at the 2009 Italian Open.
Jelena Janković was the two-time defending champion, but lost in the quarterfinals to Kuznetsova.
The top eight seeds receive a bye into the second round.