The women's 4 x 400 metres relay event at the 2009 European Athletics U23 Championships was held in Kaunas, Lithuania, at S. Dariaus ir S. Girėno stadionas (Darius and Girėnas Stadium) on 18 and 19 July.
| Gold | Anna Sedova Kseniya Zadorina Kseniya Vdovina Kseniya Ustalova Lyudmila Mochalina | |
| Silver | Esther Cremer Jill Richards Janin Lindenberg Sorina Nwachukwu Lena Schmidt | |
| Bronze | Maryna Liboza Katsiaryna Mishyna Hanna Tashpulatava Alena Kievich Maryia Kavaliova |
Competed in heat.
19 July
| Rank | Nation | Competitors | Time | Notes | ||
|---|---|---|---|---|---|---|
| align=left |  | 3:27.59 | ||||
| align=left | 3:29.21 | |||||
| align=left |  | 3:30.45 | ||||
| 4 | align=left |  | 3:30.78 | |||
| 5 | align=left |  | 3:32.92 | |||
| 6 | align=left |  | 3:33.49 | |||
| 7 | align=left |  | 3:38.06 | |||
| 8 | align=left |  | 3:38.43 |
18 July
Qualified: first 3 in each heat 2 best to the Final
| Rank | Nation | Competitors | Time | Notes | ||
|---|---|---|---|---|---|---|
| 1 | align=left | | 3:30.48 | Q | ||
| 2 | align=left | | 3:34.21 | Q | ||
| 3 | align=left | 3:34.58 | Q | |||
| 4 | align=left | | 3:35.83 | q | ||
| 5 | align=left | | 3:36.94 | q |
| Rank | Nation | Competitors | Time | Notes | ||
|---|---|---|---|---|---|---|
| 1 | align=left | | 3:38.18 | Q | ||
| 2 | align=left | | 3:38.57 | Q | ||
| 3 | align=left | | 3:39.11 | Q | ||
| 4 | align=left |  | 3:40.69 | |||
| 5 | align=left |  | 3:50.39 |
According to an unofficial count, 45 athletes from 10 countries participated in the event.
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