The 2009 ECM Prague Open was a tennis tournament played on outdoor clay courts. It was the 8th edition of the ECM Prague Open, and was part of the WTA International tournaments of the 2009 WTA Tour. It took place in Prague, Czech Republic, from July 13 through July 19, 2009.
The tournament included tennis exhibition involving Pat Cash, Mansour Bahrami and Henri Leconte.
| Player | Nationality | Ranking* | Seeding |
|---|---|---|---|
| Francesca Schiavone |  Italy | 28 | 1 |
| Sybille Bammer |  Austria | 30 | 2 |
 Czech Republic | 31 | 3 | |
| Carla Suárez Navarro |  | 32 | 4 |
| Alona Bondarenko |  Ukraine | 36 | 5 |
| Magdaléna Rybáriková |  Slovakia | 46 | 6 |
| Czech Republic | 56 | 7 | |
| Petra Kvitová | Czech Republic | 58 | 8 |
The following players received wildcards into the singles main draw
The following players received entry from the qualifying draw:
See main article: 2009 ECM Prague Open – Singles. Sybille Bammer defeated Francesca Schiavone, 7–64, 6–2
See main article: 2009 ECM Prague Open – Doubles. Alona Bondarenko / Kateryna Bondarenko defeated Iveta Benešová / Barbora Záhlavová-Strýcová, 6–1, 6–2
