The 2009 Copa Petrobras Santiago was a professional tennis tournament played on outdoor red clay courts. It was part of the 2009 ATP Challenger Tour. It took place in Santiago, Chile between 19 and 25 October 2009.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Argentina | Juan Ignacio Chela | 97 | 1 | |
| Paul Capdeville | 98 | 2 | ||
 Spain | Santiago Ventura | 105 | 3 | |
| Nicolás Massú | 118 | 4 | ||
| Spain | Rubén Ramírez Hidalgo | 124 | 5 | |
 Ecuador | Nicolás Lapentti | 125 | 6 | |
| Argentina | Sergio Roitman | 132 | 7 | |
| Argentina | Sebastián Decoud | 151 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
Santiago Ventura (retired due to abdominal pain)See main article: 2009 Copa Petrobras Santiago – Singles. Eduardo Schwank def. Nicolás Massú, 6–2, 6–2
See main article: 2009 Copa Petrobras Santiago – Doubles. Diego Cristín / Eduardo Schwank def. Juan Pablo Brzezicki / David Marrero, 6–4, 7–5
