The 2009 Copa Petrobras São Paulo was a professional tennis tournament played on outdoor red clay courts. It was the sixth edition of the tournament which was part of the 2009 ATP Challenger Tour. It took place in São Paulo, Brazil between 26 October and 1 November 2009.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Brazil | Thomaz Bellucci | 45 | 1 | |
 Argentina | Juan Ignacio Chela | 94 | 2 | |
| Paul Capdeville | 95 | 3 | ||
 Spain | Santiago Ventura | 117 | 4 | |
 Ecuador | Nicolás Lapentti | 122 | 5 | |
| Rui Machado | 123 | 6 | ||
| Nicolás Massú | 143 | 7 | ||
| Brazil | Thiago Alves | 149 | 8 |
The following players received wildcards into the singles main draw:
Thiago Alves Marcelo Demoliner Nicolás Lapentti Gabriel WanderleyThe following player received a Special Exempt into the singles main draw:
The following players received entry from the qualifying draw:
See main article: 2009 Copa Petrobras São Paulo – Singles. Thomaz Bellucci def. Nicolás Lapentti, 6–4, 6–4
See main article: 2009 Copa Petrobras São Paulo – Doubles. Franco Ferreiro / Ricardo Mello def. Diego Junqueira / David Marrero, 6–3, 6–3
