The 2009 Copa Petrobras Asunción was a professional tennis tournament played on outdoor red clay courts. It was the fourth edition of the tournament which was part of the 2009 ATP Challenger Tour. It took place in Asunción, Paraguay between 12 and 18 October 2009.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Argentina | Máximo González | 68 | 1 | |
| Nicolás Massú | 92 | 2 | ||
| Paul Capdeville | 95 | 3 | ||
 Spain | Santiago Ventura | 108 | 4 | |
| Spain | Daniel Gimeno-Traver | 112 | 5 | |
| Spain | Rubén Ramírez Hidalgo | 125 | 6 | |
 France | Laurent Recouderc | 127 | 7 | |
| Blaž Kavčič | 148 | 8 |
The following players received wildcards into the singles main draw:
Mariano PuertaThe following player received a Special Exempt into the singles main draw:
The following players received entry from the qualifying draw:
Facundo Bagnis
Marcelo Demoliner Guido Pella Carles Poch-GradinSee main article: 2009 Copa Petrobras Asunción – Singles. Ramón Delgado def. Daniel Gimeno-Traver, 7–6(2), 1–6, 6–3
See main article: 2009 Copa Petrobras Asunción – Doubles. Rubén Ramírez Hidalgo / Santiago Ventura def. Máximo González / Eduardo Schwank, 6–3, 0–6, [10–8]