| Date: | August 31 – September 6 |
| Edition: | 4th |
| Location: | Como, Italy |
| Champs: |  Oleksandr Dolgopolov Jr. |
| Champd: |  Marco Crugnola / Alessandro Motti |
The 2009 Città di Como Challenger was a professional tennis tournament played on outdoor red clay courts. It was the fourth edition of the tournament which was part of the 2009 ATP Challenger Tour. It took place in Como, Italy between 31 August and 6 September 2009.
| Nationality | Player | Ranking* | Seeding | |
|---|---|---|---|---|
| Italy | Paolo Lorenzi | 136 | 1 | |
| Kevin Anderson | 148 | 2 | ||
 France | Alexandre Sidorenko | 153 | 3 | |
 Argentina | Eduardo Schwank | 166 | 4 | |
| Italy | Tomas Tenconi | 170 | 5 | |
| France | Olivier Patience | 181 | 6 | |
| Ukraine | Oleksandr Dolgopolov Jr. | 192 | 7 | |
| Italy | Marco Crugnola | 199 | 8 |
The following players received wildcards into the singles main draw:
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
See main article: 2009 Città di Como Challenger – Singles. Oleksandr Dolgopolov Jr. def. Juan-Martín Aranguren, 7–5, 7–6(5)
See main article: 2009 Città di Como Challenger – Doubles. Marco Crugnola / Alessandro Motti def. Treat Conrad Huey / 
Harsh Mankad, 7–6(3), 6–3
