The 2009 Bancolombia Open was a professional tennis tournament played on outdoor red clay courts. It was part of the 2009 ATP Challenger Tour. It took place in Bogotá, Colombia between 16 and 22 March 2009.
| Nationality | Player | Ranking* | Seeding | |
|---|---|---|---|---|
 Spain | Óscar Hernández | 67 | 1 | |
| Frederico Gil | 79 | 2 | ||
 Argentina | Brian Dabul | 82 | 3 | |
| Spain | Iván Navarro | 83 | 4 | |
| Argentina | Máximo González | 96 | 5 | |
| Argentina | Leonardo Mayer | 97 | 6 | |
| Argentina | Sergio Roitman | 103 | 7 | |
 Brazil | Thiago Alves | 123 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
Juan Pablo Amado Diego Álvarez
Riccardo GhedinSee main article: 2009 Bancolombia Open – Singles. Horacio Zeballos def. 
Santiago González, 7–6(3), 6–0
See main article: 2009 Bancolombia Open – Doubles. Sebastián Prieto / Horacio Zeballos def. 
Alexander Peya / Fernando Vicente, 4–6, 6–1, [11–9]
