The 2009 Alessandria Challenger (known as 2009 Trofeo Cassa di Risparmio di Alessandria) was a professional tennis tournament played on outdoor red clay courts. It was part of the 2009 ATP Challenger Tour. It took place in Alessandria, Italy between May 25–31, 2009.
| Nationality | Player | Ranking* | Seeding | |
|---|---|---|---|---|
 Spain | Rubén Ramírez Hidalgo | 131 | 1 | |
| Jesse Levine | 142 | 2 | ||
 Brazil | Ricardo Hocevar | 159 | 3 | |
| Spain | David Marrero | 166 | 4 | |
 Ecuador | Giovanni Lapentti | 172 | 5 | |
 Colombia | Alejandro Falla | 173 | 6 | |
 Italy | Paolo Lorenzi | 174 | 7 | |
| Spain | Daniel Muñoz de la Nava | 175 | 8 |
The following players received wildcards into the singles main draw:
Laurynas Grigelis Jacopo Marchegiani Rubén Ramírez Hidalgo Simone VagnozziThe following players received entry from the qualifying draw:
See main article: 2009 Alessandria Challenger – Singles. Blaž Kavčič def. 
Jesse Levine, 7–5, 6–3
See main article: 2009 Alessandria Challenger – Doubles. Rubén Ramírez Hidalgo / José Antonio Sánchez de Luna def. 
Martín Alund / Guillermo Hormazábal, 6–4, 6–2
