| Competition: | Cypriot Third Division |
| Season: | 2009–10 |
| Winners: | Chalkanoras (2nd title) |
| Promoted: | Chalkanoras Adonis Anagennisi |
| Relegated: | THOI Kissos Achyronas |
| League Topscorer: | Alexandre Soares (18 goals) |
| Matches: | 182 |
| Total Goals: | 544 |
| Prevseason: | 2008–09 |
| Nextseason: | 2010–11 |
The 2009–10 Cypriot Third Division was the 39th season of the Cypriot third-level football league. Chalkanoras Idaliou won their 2nd title.
Fourteen teams participated in the 2009–10 Cypriot Third Division. All teams played against each other twice, once at their home and once away. The team with the most points at the end of the season crowned champions. The first three teams were promoted to the 2010–11 Cypriot Second Division and the last three teams were relegated to the 2010–11 Cypriot Fourth Division.
Teams received three points for a win, one point for a draw and zero points for a loss.
Teams promoted to 2009–10 Cypriot Second Division
Teams relegated from 2008–09 Cypriot Second Division
Teams promoted from 2008–09 Cypriot Fourth Division
Teams relegated to 2009–10 Cypriot Fourth Division
| Club | Venue |
|---|---|
| AEZ | Zakaki Municipal Stadium |
| AEK | Chloraka Municipal Stadium |
| Adonis | Adonis Idaliou Stadium |
| Anagennisi | Anagennisi Football Ground |
| Achyronas | Liopetri Municipal Stadium |
| Digenis | Xylotympou Municipal Stadium |
| Elpida | Michalonikion Stadium |
| Ethnikos | Stadium Keryneia Epistrophi |
| ENAD | Poli Chrysochous Municipal Stadium |
| THOI | EN THOI Stadium |
| Kissos | Kissonerga Municipal Stadium |
| Iraklis | Kykkos Stadium |
| Spartakos | Kiti Municipal Stadium |
| Chalkanoras | Chalkanoras Stadium |