The 2009-10 version of the Syrian Cup is the 40th edition to be played. It is the premier knockout tournament for football teams in Syria.
Al-Karamah went into this edition as the holders.
The cup winner were guaranteed a place in the 2011 AFC Cup.
32 teams play a knockout tie. 16 clubs advance to the next round. Games played over two legs
The games were played on May 10–19, 2010.|}¹ Azaz failed to the 2nd leg, match awarded 3-0 to Al-Nawair.
² Ommal Al-Quneitra failed to the First round, matches awarded 3–0 to Afrin.
16 teams play a knockout tie. 8 clubs advance to the next round. Games played over two legs
The games were played on May 18–26, 2010.|}¹ Hutteen failed to the 2nd leg, match awarded 3–0 to Al-Jazeera.
8 teams play a knockout tie. 4 clubs advance to the next round. Games played over two legs
The games were played on May 28–1 June 2010.|}
4 teams played a knockout tie. 2 clubs advanced to the Final. Games played over two legs.
The games were played on June 4–08, 2010.|}