The 2010 Segunda División B play-offs (Playoffs de Ascenso or Promoción de Ascenso) were the final playoffs for promotion from 2009–10 Segunda División B to the 2010–11 Segunda División. The four first placed teams in each of the four Segunda División B groups played the Playoffs de Ascenso and the four last placed teams in Segunda División were relegated to Segunda División B. It also decided the two teams which placed 16th to be relegated to the 2010–11 Tercera División.
The four group winners had the opportunity to promote directly and become the overall Segunda División B champion. The four group winners were drawn into a two-legged series where the two winners promoted to the Liga Adelante and entered into the final for the Segunda División B champion. The two losing semifinalists entered the playoff round for the last two promotion spots.
The four group runners-up were drawn against one of the three fourth-placed teams outside their group while the four third-placed teams were drawn against each other in a two-legged series. The six winners advanced with the two losing semifinalists to determine the four teams that entered the last two-legged series for the last two promotion spots. In all the playoff series, the lower-ranked club played at home first. Whenever there was a tie in position (e.g. like the group winners in the semifinal round and final or the third-placed teams in the first round), a draw determined the club to play at home first.
The draw were held in the RFEF headquarters, in Las Rozas (Madrid), on 10 May 2010, 16:30 CEST.
| Group | Team |
|---|---|
| 1 | Ponferradina |
| 2 | Alcorcón |
| 3 | Sant Andreu |
| 4 | Granada |
|}The aggregate winners will be promoted and qualified to the 2010–11 Segunda División B Final. The aggregate losers will be relegated to the Non-champions promotion play-off Second Round.
|}
| Group | Position | Team |
|---|---|---|
| 1 | 2nd | Eibar |
| 2 | 2nd | Oviedo |
| 3 | 2nd | Barcelona B |
| 4 | 2nd | Melilla |
| 1 | 3rd | Palencia |
| 2 | 3rd | Guadalajara |
| 3 | 3rd | Ontinyent |
| 4 | 3rd | Real Jaén |
| 1 | 4th | Pontevedra |
| 2 | 4th | Universidad LPGC |
| 3 | 4th | Alcoyano |
| 4 | 4th | Poli Ejido |
|}
| Group | Position | Team | Notes |
|---|---|---|---|
| 2 | 1st | Alcorcón | Relegated from Group Winners promotion play-off |
| 3 | 1st | Sant Andreu | Relegated from Group Winners promotion play-off |
| 1 | 4th | Pontevedra | |
| 4 | 3rd | Real Jaén | |
| 2 | 4th | Universidad LPGC | |
| 1 | 2nd | Eibar | |
| 3 | 3rd | Ontinyent | |
| 3 | 2nd | Barcelona B | |
|}
| Group | Position | Team |
|---|---|---|
| 3 | 1st | Sant Andreu |
| 3 | 3rd | Ontinyent |
| 2 | 1st | Alcorcón |
| 3 | 2nd | Barcelona B |
|}
| Group | Team |
|---|---|
| 1 | Guijuelo |
| 2 | Toledo |
| 3 | Espanyol B |
| 4 | Roquetas |
|}
The aggregate losers will be relegated to Tercera División.