| Competition: | Macedonian Second League |
| Season: | 2009–10 |
| Winners: | Shkëndija |
| Promoted: | Shkëndija Skopje Napredok Bregalnica Shtip |
| Relegated: | None |
| Prevseason: | 2008–09 |
| Nextseason: | 2010–11 |
The 2009–10 Macedonian Second Football League was the eighteenth season since its establishment. It began on 15 August 2009 and ended on 19 May 2010.[1] Due to the expansion of the league, no teams were relegated that season.
| Club | City | Stadium | Capacity | |
|---|---|---|---|---|
| 11 Oktomvri | Prilep | Stadion Goce Delchev | 15,000 | |
| Belasica | Strumica | Stadion Mladost | 6,370 | |
| Bregalnica 2008 | Shtip | Gradski stadion Shtip | 4,000 | |
| Cementarnica 55 | Skopje | Stadion Cementarnica | 2,000 | |
| Drita | Bogovinje | Stadion Bogovinje | 500 | |
| Lokomotiva | Skopje | Stadion Komunalec | 1,000 | |
| Miravci | Miravci | Stadion Miravci | 1,000 | |
| Napredok | Kichevo | Gradski stadion Kichevo | 5,000 | |
| Novaci 2005 | Novaci | Stadion Novaci | 500 | |
| Ohrid 2004 | Ohrid | SRC Biljanini Izvori | 3,000 | |
| Skopje | Skopje | Stadion Avtokomanda | 4,000 | |
| Shkëndija 79 | Tetovo | Gradski stadion Tetovo | 15,000 | |
| Vlaznimi | Struga | Stadion Gradska Plazha | 500 | |
| Vëllazërimi | Kichevo | Gradski stadion Kichevo | 5,000 |