| Club: | Nistru |
| Season: | 2009–10 Moldovan National Division |
| Chairman: |  Vasile Traghira |
| Manager: | Lilian Popescu |
| Mgrtitle: | Manager |
| Chrtitle: | Chairman |
| League: | Moldovan National Division |
| League Result: | 12th |
| Cup1: | Moldovan Cup |
| Cup1 Result: | Round of 16 |
| Prevseason: | 2008–09 |
| Nextseason: | 2010–11 |
2009–10 Moldovan National Division season is the 16th Moldovan National Division season in the history of FC Nistru.
Squad given according to the official website as of June 6, 2010 http://www.divizianationala.com/index.php?option=com_joomleague&func=showPlayers&p=2&ttid=27&Itemid=1