| Season: | 2009–10 |
| Relegated: | None (originally Otterup B&IK, but annulled) |
| League Topscorer: | West: Danilo Arrieta (28)[1] East: Niels Peter Kjølbye (20)[2] |
| Nextseason: | 2010–11 |
The 2009-10 season in Danish 2nd Division was divided in two groups. The two winners, Brønshøj BK and FC Hjørring were promoted to the 2010–11 Danish 1st Division, along with the runner-up from the West-division Hobro IK, after they beat their Eastern counterpart B.93 5–1 on aggregate in a two-legged promotion play-off. Relegation was decided when Otterup B&I from the West division lost 4–5 to BK Skjold in another two-legged match-up between the bottom teams from the divisions. This relegation was later annulled, as 1st Division relegatee BK Frem went bankrupt, forcing a further two-level-relegation to the Copenhagen Series and leaving an extra place open for Otterup.
On 6 March 2010 it was decided that the eight Superliga clubs' second teams should be withdrawn from the Second Division at the end of the season and be transferred to a separate reserves competition. Therefore, the relegation to the Denmark Series was reduced from six teams to one, and the promotion the other way increased from six to nine. A two-legged relegation play-off between the two 16th ranked teams would decide the relegation.
| Club | Group | Finishing position last season | First season of current spell in 2nd Divisions | |
|---|---|---|---|---|
| AaB 2 | West | 11th | 2007–08 | |
| Aarhus Fremad | West | 8th | 2008–09 | |
| AGF 2 | West | 14th | 2007–08 | |
| Allerød FK | East | 2nd in Denmark Series Pool 1 | 2009–10 | |
| B.93 | East | 2nd | 2005–06 | |
| B 1908 | East | 3rd in Denmark Series Pool 1 | 2009–10 | |
| BGA | East | 11th | 1999–00 | |
| BK Avarta | East | 8th | 2008–09 | |
| BK Skjold | East | 9th | 2006–07 | |
| Blokhus FC | West | 4th | 2002–03 | |
| Brøndby 2 | East | 4th | 2005–06 | |
| Brønshøj BK | East | 3rd | 2006–07 | |
| BSV | East | 13th | 2005–06 | |
| Esbjerg 2 | West | 5th | 2005–06 | |
| F.C. Copenhagen 2 | East | 7th | 2005–06 | |
| FC Hjørring | West | 13th | 1999–00 | |
| FC Svendborg | West | 9th | 2008–09 | |
| Greve Fodbold | East | 12th | 2006–07 | |
| HIK | East | 5th | 2008–09 | |
| Hobro IK | West | 7th | 2006–07 | |
| Holstebro BK | West | 2nd in Denmark Series Pool 3 | 2009–10 | |
| Ikast FS (FCM 2) | West | 12th | 2006–07 | |
| LFA | East | 16th in 1st Division | 2009–10 | |
| Nordvest FC | East | 2nd in Denmark Series Pool 2 | 2009–10 | |
| Næsby BK | West | 15th | 2004–05 | |
| OB 2 | West | 2nd | 2007–08 | |
| Otterup B&I | West | 1st in Denmark Series Pool 2 | 2009–10 | |
| Randers Freja (RFC 2) | West | 6th | 2008–09 | |
| Stenløse BK | East | 6th | 2005–06 | |
| Tjørring IF | West | 6th in Denmark Series Pool 3 | 2009–10 | |
| Vanløse IF | East | 14th | 2007–08 | |
| Varde IF | West | 10th | 2005–06 |
Hobro IK win 5 – 1 on aggregate and are promoted to the 2010–11 Danish 1st Division
The relegation of Otterup B&I for losing this playoff was later cancelled, as BK Frem went bankrupt and left another place open in the 2nd Division.
Otterup B&I lose 4 – 5 on aggregate and are relegated to the 2010–11 Danish Series (later annulled)