| Competition: | Cypriot Fourth Division |
| Winners: | EN Parekklisia (1st title) |
| Promoted: | EN Parekklisia N.&S. Erimis Anagennisi G. |
| Relegated: | Orfeas Ellinismos Olympos AEK |
| Matches: | 210 |
| Total Goals: | 692 |
| League Topscorer: | Constantinos Constantinou (27 goals) |
| Prevseason: | 2008–09 |
| Nextseason: | 2010–11 |
| Season: | 2009–10 |
The 2009–10 Cypriot Fourth Division was the 25th season of the Cypriot fourth-level football league. Enosis Neon Parekklisia won their 1st title.
Fifteen teams participated in the 2009–10 Cypriot Fourth Division. All teams played against each other twice, once at their home and once away. The team with the most points at the end of the season crowned champions. The first three teams were promoted to the 2010–11 Cypriot Third Division and the last four teams were relegated to regional leagues.
Teams received three points for a win, one point for a draw and zero points for a loss.
Teams promoted to 2009–10 Cypriot Third Division
Teams relegated from 2008–09 Cypriot Third Division
Teams promoted from regional leagues
Teams relegated to regional leagues
Notes:
| Club | Venue |
|---|---|
| AEK Kythreas | Kykkos Stadium |
| Anagennisi Germasogeias | Germasogeia Municipal Stadium |
| Anagennisi Trachoniou | Trachoni Municipal Stadium |
| ASPIS Pylas | Pyla Municipal Stadium |
| Ethnikos Latsion FC | Latsia Municipal Stadium |
| Ellinismos Akakiou | Ellinismos Akakiou Stadium |
| Enosis Neon Parekklisia | Parekklisia Municipal Stadium |
| Enosis Kokkinotrimithia | Kokkinotrimithia Municipal Stadium |
| Karmiotissa Pano Polemidion | Pano Polemidia Municipal Stadium |
| Konstantios & Evripidis Trachoniou | Trachoni Municipal Stadium |
| Nikos & Sokratis Erimis | Erimi Municipal Stadium |
| Olympos Xylofagou | Makario Stadium Xylofagou |
| Orfeas Nicosia | Orfeas Stadium |
| P.O. Xylotymvou | Xylotympou Municipal Stadium |
| Ormideia FC | Ormideia Municipal Stadium |