The 2008-09 season in Danish 2nd Division was divided in two groups. The two winners were promoted to the 2009–10 Danish 1st Division, together with the winner of a promotion game between the two runners-up. Second squad teams are ineligible for promotion.
| Club | Group | Finishing position last season | First season of current spell in 2nd Divisions | |
|---|---|---|---|---|
| AaB 2 | West | 6th | 2007–08 | |
| Aarhus Fremad | West | 15th in 1st Division | 2008–09 | |
| AGF 2 | West | 5th | 2007–08 | |
| B.93 | East | 9th | 2005–06 | |
| BK Avarta | East | 1st in Denmark Series Pool 1 | 2008–09 | |
| BK Skjold | East | 8th | 2006–07 | |
| Blokhus FC | West | 10th | 2002–03 | |
| Brabrand IF | West | 2nd | 2007–08 | |
| Brøndby 2 | East | 4th | 2005–06 | |
| Brønshøj BK | East | 3rd | 2006–07 | |
| BSV | East | 13th | 2005–06 | |
| Esbjerg 2 | West | 7th | 2005–06 | |
| F.C. Copenhagen 2 | East | 6th | 2005–06 | |
| FC Fyn | West | 3rd | 2004–05 | |
| FC Hjørring | West | 13th | 1999–00 | |
| FC Svendborg | West | 2nd in Denmark Series Pool 1 | 2008–09 | |
| FC Vestsjælland | East | 5th | 2002–03 | |
| Glostrup FK | East | 11th | 1999–00 | |
| Greve Fodbold | East | 10th | 2006–07 | |
| HIK | East | 14th in 1st Division | 2008–09 | |
| Hobro IK | West | 9th | 2006–07 | |
| Ikast FS (FCM 2) | West | 8th | 2006–07 | |
| Næsby BK | West | 14th | 2004–05 | |
| OB 2 | West | 4th | 2007–08 | |
| Randers Freja (RFC 2) | West | 1st in Denmark Series Pool 2 | 2008–09 | |
| Skovbakken IK | West | 5th in Denmark Series Pool 2 | 2008–09 | |
| Skovlunde IF | East | 7th | 2006–07 | |
| Stenløse BK | East | 12th | 2005–06 | |
| Vanløse IF | East | 12th | 2007–08 | |
| Varde IF | West | 11th | 2005–06 | |
| Værløse BK | East | 14th | 2007–08 | |
| Ølstykke FC | East | 16th in 1st Division | 2008–09 |
Last updated: 9 June 2009; Source: Danish Football Association
| Pos | Player | Scored for | Goals |
|---|---|---|---|
| 1 |  Thomas Wagner | Stenløse BK | 23 |
| 2 | Rasmus Festersen | FC Vestsjælland | 20 |
| 3 | Patrick Mortensen | Brøndby 2 | 19 |
| 4 | Danni König | Brønshøj BK | 16 |
| 5 | Andreas Mortensen | FC Vestsjælland | 14 |
| 6 | Jesper Fosgaard | BK Avarta | 13 |
| Kevin Bechmann Timm | F.C. Copenhagen 2 | ||
| 8 | Christian Lundby | Greve Fodbold | 12 |
| Morten Seifert | BK Avarta | ||
| 10 | Geert Baunsgaard | B.93 | 11 |
| Christian Brøndum | Stenløse BK | ||
| Thomas Christiansen | F.C. Copenhagen 2 | ||
| Kristian Krog | Vanløse IF | ||
| Emil Sørensen | Ølstykke FC | ||
| Mads Torry | Glostrup FK | ||
| Thomas Myssing | BK Søllerød-Vedbæk |
Last updated: 9 June 2009; Source: Danish Football Association
| Pos | Player | Scored for | Goals |
|---|---|---|---|
| 1 | Jesper Rasmussen | FC Svendborg | 20 |
| 2 | Jacob Bymar | Varde IF | 19 |
| 3 | Nikolaj Thomsen | Blokhus FC | 17 |
| 4 | Mads Overgaard | FC Fyn | 16 |
| 5 | Peter Tranberg | Blokhus FC | 14 |
| Martin Laustsen | Brabrand IF | ||
| 7 | Brian Johansen | Aarhus Fremad | 12 |
| Ronnie Schwartz | AaB 2 | ||
| Mark Tubæk | OB 2 | ||
| 10 | Christian Andreasen | Randers Freja | 11 |
| Quincy Antipas | Blokhus FC | ||
| Martin Koordt | Næsby BK | ||
| Lennart Kruse | Varde IF | ||
| Lwasi Saluha | FC Fyn | ||
| Pelle Weber | OB 2 |
The two runners-up will play promotion game on home and away basis.
The two teams placed 15th in each group will play relegation game on home and away basis.