The women's 4 × 400 metres relay at the 2007 World Championships in Athletics was held at the Nagai Stadium on 1 and 2 September.
| width=200 valign=top | DeeDee Trotter Allyson Felix Mary Wineberg Sanya Richards Monique Hennagan* Natasha Hastings* | width=200 valign=top | Shericka Williams Shereefa Lloyd Davita Prendergast Novlene Williams Anastasia Le-Roy* | width=200 valign=top | Christine Ohuruogu Marilyn Okoro Lee McConnell Nicola Sanders Donna Fraser* |
* Runners who participated in the heats only and received medals.
| Date | Time | Round | |
|---|---|---|---|
| September 1, 2007 | 20:05 | Heats | |
| September 2, 2007 | 20:30 | Final |
Qualification: First 3 of each heat (Q) plus the 2 fastest times (q) advance to the final.
| Rank | Heat | Nation | Athletes | Time | Notes |
|---|---|---|---|---|---|
| 1 | 2 | 3:23.37 | Q, | ||
| 2 | 1 | 3:23.49 | Q, | ||
| 3 | 1 | 3:25.14 | Q | ||
| 4 | 2 | 3:25.45 | Q, | ||
| 5 | 1 | 3:26.14 | Q, | ||
| 6 | 2 | 3:26.45 | Q | ||
| 7 | 1 | 3:27.04 | q, | ||
| 8 | 2 | 3:27.14 | q, NR | ||
| 1 | 3:27.97 | ||||
| 10 | 2 | 3:29.87 | |||
| 11 | 2 | 3:30.17 | NR | ||
| 12 | 1 | 3:30.76 | |||
| 13 | 2 | 3:34.34 | |||
| 14 | 1 | 3:35.69 | |||
| 2 | |||||
| Rank | Nation | Athletes | Time | Notes | |
|---|---|---|---|---|---|
| 3:18.55 | |||||
| Shericka Williams, Shereefa Lloyd, Davita Prendergast, Novlene Williams | 3:19.73 | NR | |||
| 3:20.04 | NR | ||||
| 3:20.25 | |||||
| 3:21.88 | NR | ||||
| 3:26.49 | |||||
| 3:27.05 | |||||
| 3:29.14 |