See main article: 2007 Sony Ericsson Open.
Serena Williams defeated Justine Henin in the final, 0–6, 7–5, 6–3 to win the women's singles tennis title at the 2007 Miami Open. She saved two championship points en route to the title, being down in the final 0-6, 4-5 15-40.
All seeds received a bye into the second round.