Justine Henin defeated Jelena Janković in the final, 7–6(7–3), 7–5 to win the women's singles tennis title at the 2007 Canadian Open.
Ana Ivanovic was the defending champion, but lost in the second round to Yan Zi.
The top eight seeds receive a bye into the second round.
Justine Henin (champion)
Jelena Janković (final)
Svetlana Kuznetsova (quarterfinals) Ana Ivanovic (second round) Anna Chakvetadze (second round, retired due to a viral illness) Nadia Petrova (quarterfinals)
Marion Bartoli (quarterfinals, retired due to a viral illness) Elena Dementieva (second round) Dinara Safina (third round)
Shahar Pe'er (third round) Tatiana Golovin (semifinals)
Sybille Bammer (third round)
Alona Bondarenko (first round)
Lucie Šafářová (second round)