See main article: 2007 Italian Open (tennis).
Jelena Janković defeated Svetlana Kuznetsova in the final, 7–5, 6–1 to win the women's singles tennis title at the 2007 Italian Open. It was her second Tier I title.
Martina Hingis was the reigning champion, but did not participate this year due to a hip injury.
The top eight seeds received a bye into the second round.